StarBright Blue 700 Secondary Antibody Range Instructions for use, tips, troubleshooting and further information are available in the StarBright Blue Antibodies instruction manual and Comprehensive Guide to Fluorescence Western Blotting instruction manual. minutes)Įasy multiplexing options - compatible with stain-free gel and blot imaging detection and hFAB Rhodamine Antibodies as well as antibodies labeled with traditional fluorophores Short exposure times - 50- to 100-fold shorter (seconds vs. Highly sensitive fluorescent detection - 2- to 4-fold lower limit of detection than traditional fluorophores Learn more about the validation process and customers’ experience using PrecisionAb Antibodies and the full range available.Īdvantages of StarBright Blue Secondary Antibodies compared to other traditional fluorophores: PrecisionAb Antibodies were chosen for this multiplex western blot because of their superior performance and reproducibility, having been rigorously tested and selected for high specificity, high sensitivity, and consistency. Composite blot image for quadruple western blotting.įigure 1 shows a composite blot image with four distinct proteins visualized using StarBright Blue 520 and StarBright Blue 700 Secondary Antibodies, in combination with Dylight 800 and a rhodamine labeled recombinant human hFAB antibody, for the detection of popular housekeeping proteins on the ChemiDoc MP Imaging System. They are excited with blue light (440–470 nm) and are available in two colors: StarBright Blue 520 emits in the green range of the spectrum (emission maximum: 520 nm) and StarBright Blue 700 maximally emits in the far red/near infrared region of the spectrum (700 nm).įig. StarBright Dyes’ fluorescence emission is restricted to a narrow spectral band, making them ideal for multiplex fluorescence imaging for western blotting. Labeled with exceptionally bright and stable semiconducting nanoparticles. Read the introductory guide for detailed information on multiplex fluorescent western blotting. Imaging with ChemiDoc MP Imaging System.hFAB Rhodamine Anti-Housekeeping Protein Primary Antibodies.Fluorescent StarBright Blue 700 and 520 Secondary Antibodies.Greater consistency of readout using fluorescent dyes rather than the variability of an enzymatic reactionĪmount of information collected is maximized, using as little of your rare/precious sample as possibleīio-Rad offers a new generation of multiplex western blotting reagents and equipment, that includes: Results in context by simultaneous analysis of several proteins and parameters Multiple samples are analyzed under the same experimental conditions The benefits of fluorescent western blotting multiplexing are: The increasing need to maximize data from a single sample has led to the development of fluorescent based multiplexing assays. Multiplex fluorescent western blotting enables the simultaneous analysis of multiple proteins in a single sample on the same blot, obtaining as much information as possible in one experiment.
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